Details

Project TitleMonoclonal Antibody Specific for Tau Protein Nitrated at Tyrosine 29
Track Code2007-006
Short Description

Tau-nY29 is a mouse monoclonal antibody (mAb) specific for human tau wherein the Tyrosine residue at position 29 has been nitrated.

Abstract

The antibody is of the IgG1 subtype.  The antibody detects soluble tau and paired helical filament tau from severely affected Alzheimer’s brain but fails to recognize tau from a normal aged brain.

The Tau-nY29 mAb is a powerful tool for detection soluble human tau with a nitro group attached at amino acid Tyr29. The nitration of Tyr 29 is indicative of neurodegenerative diseases, including Alzheimer’s.  Therefore, this antibody is useful in studying the role of nitration of tau in Alzheimer’s disease.

The Tau-nY29 mAb was derived from a hybridoma cell line created through the fusion of SP2/o myeloma cells with splenocytes from a mouse immunized with a synthetic peptide corresponding to human tau amino acids 25-37 that was nitrated at Tyr29.  The hybridoma cell line was subcloned four times and stored in frozen liquid nitrogen.  Tau-nY29 is specific for human tau constructs nitrated at Tyr29.  It neither reacts with non-nitrated peptides nor human tau proteins nitrated at other tyrosine residues as demonstrated by ELISA and immunoblot assays.

 
Tagsdiagnostic tools, healthcare, neurology, antibodies, research tools
 
Posted DateMar 18, 2011 3:23 PM

Inventor(s)

Lester Binder 

Advantages

Applications

Status

This antibody is available to be licensed as a research reagent.

Further information regarding this antibody is available in the following reference: Tau nitration occurs at tyrosine 29 in the fibrillar lesions of Alzheimer's disease and other tauopathies. Reynolds, et al. Journal of Neuroscience. 2006. 26(42):10636-45

Contact Information

Gwendolyn Humphreys, PhD

Invention Associate

(p) 847-467-0308

(e) Gwendolyn.Humphreys@northwestern.edu