Details

Project TitleTau-13 Monoclonal Antibody
Track Code2000-081
Short Description

Tau-13 is a mouse monoclonal antibody (mAb) that binds to human tau as demonstrated by ELISA and immunoblot assays and is able to stain brain tissue early in Alzheimer's disease.

Abstract

The Tau-13 mAb would be useful to researchers who wish to detect human tau in solid phase assays as it has a remarkably high affinity for the immunogen. This antibody is useful in tissue staining and may be used for pull-down assays.

The Tau-13 mAb was derived from a hybridoma cell line created through the fusion of SP2/o myeloma cells with splenocytes from a mouse immunized with the polymeric form of the longest human tau isoform. The hybridoma cell line was subcloned four times and stored in frozen liquid nitrogen. Tau-13 is specific for human tau; it does not react with bovine, murine or rat tau. The antibody is of the IgG1 subtype. Preliminary assignment of its epitope indicates that it maps to amino acid residues 2-18 on the longest isoform of human tau.

 
Tagsdiagnostic tools, neurology, research tools, antibodies
 
Posted DateMar 18, 2011 2:56 PM

Inventor(s)

Lester Binder 

Applications

Advantages

The Tau-13 mAb was derived from a hybridoma cell line created through the fusion of SP2/o myeloma cells with splenocytes from a mouse immunized with the polymeric form of the longest human tau isoform. The hybridoma cell line was subcloned four times and stored in frozen liquid nitrogen. Tau-13 is specific for human tau; it does not react with bovine, murine or rat tau. The antibody is of the IgG1 subtype. Preliminary assignment of its epitope indicates that it maps to amino acid residues 2-18 on the longest isoform of human tau.

Status

This antibody is available to be licensed as a research reagent.

Contact Information

Gwendolyn Humphreys, PhD

Invention Associate

(p) 847-467-0308

(e) Gwendolyn.Humphreys@northwestern.edu