Details

Project TitleImproved Transformation of Clostridium
Track Code2000-090
Short Description

Method for methylation of DNA to ensure transformation of Clostridium acetobutylicum

#researchtool #reagent

Abstract

Northwestern researchers have designed a plasmid that expresses a methyltransferase from Bacillus subtilis in Escherichia coli. This plasmid, pAN1, can be co-expressed with genes from C. acetobutylicum to methylate them, protecting them against degradation by C. acetobutylicum nucleases upon transformation. C. acetobutylicum is a well-studied solventogenic bacterium with many industrial applications. However, it expresses many nucleases that interfere with introduction of plasmids, so it has been difficult to generate genetic mutants or deletions that could improve solvent production by this bacterium. Thus, this innovative reagent from the Papoutsakis laboratory will quicken the pace of basic genetic research of C. acetobutylicum with potentially significant implications for solvent production by this bacterium.

 
Tagsresearch tool: reagent
 
Posted DateApr 24, 2015 10:29 AM

Inventor(s)

Lee Mermelstein

Eleftherios Papoutsakis*

Applications

  • Introduction of plasmids into C. acetobutylicum cells
  • Solvent production by C. acetobutylicum

Advantages

  •  Simpler and faster compared to current alternatives

Publications

IP Status

Marketing Contact

Becky Crump, PhD

Associate Director

(p) 847. 491.3630

(e) beckyc@northwestern.edu