Details

Project TitleC. acetobutylicum Gene Expression Reporter Plasmids
Track Code2000-091
Short Description

New reporter plasmids for study of clostridial gene promoters

#research tool #reagent

Abstract

Northwestern researchers designed a novel reporter plasmid that enables research of gene expression in Clostridium acetobutylicum. This bacterial genus is well known for its use in fuel fermentation, but little is known about how solvent production pathways are regulated. To address this problem, Dr. Papoutsakis and colleagues exploited a well-known reporter gene LacZ, whose expression is controlled by three different C. acetobutylicum promoters from the solventogenic metabolic pathways. Expression of LacZ gene is measured by a simple and sensitive β-galactosidase assay, which is routinely performed in many laboratories around the world. In addition, the strength or activation of any other clostridial promoter can be tested in the same manner.

 
Tagsresearch tool: reagent
 
Posted DateApr 23, 2015 5:04 PM

Inventor(s)

Eleftherios Papoutsakis*

Seshu Tummala

Neil Welker

Applications

  • Research reagent

Advantages

  • Sensitive
  • Simple to use
  • Versatile- promoters can be replaced

Publications

IP Status

Marketing Contact

Becky Crump, PhD

Associate Director

(p) 847.491.3630

(e) beckyc@northwestern.edu