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Project TitleLuciferase Reporter for Heat Shock Protein Promoter
Track Code2009-004
Short Description

 Novel reagent for detection of heat shock protein response

#researchtool #gene

Abstract

A Northwestern University researcher designed a reporter plasmid for quantitative determination of heat shock protein (HSP) response in a variety of cell lines. HSP response has been implicated as a mechanism of cell protection from various insults, and it is thought that its stimulation can lead to treatments for several diseases. The reporter plasmid generated in the Morimoto lab couples a luciferase gene to the HSP70.1 promoter region, so that stimulation of the HSP response leads to an increase in luminescence production. This system can be used for screening compound libraries for their ability to activate or inhibit HSP in several cell lines, in both transient and stable transfection.

 
TagsRESEARCH TOOL: gene
 
Posted DateApr 23, 2015 4:52 PM

Inventor(s)

Richard I. Morimoto

Applications

  • Quantitative determination of HSP response
  • High throughput screening for HSP activators

Advantages

  • Able to respond to various HSP inducing conditions
  • Compatible with various cell lines (HeLa, MCF7, BT474, H157, SH-SY5Y)

Publications

IP Status

Marketing Contact

Becky Crump

Associate Director

(p) 847.491.3630

(e) beckyc@northwestern.edu