Project TitleReverse Transfection from Solid Scaffolds
Track Code2003-090
Short Description

A controlled, efficient method to deliver nucleic acid complexes to cells from a supporting scaffold

#researchtool #reagent #therapeutic #genetherapy


Northwestern University researchers have developed a reversed transfection method, wherein nucleic acids are delivered to cells from the substrate that supports them. Introduction of nucleic acids into mammalian cells has been an important tool for molecular biology and genomics research as well as for development of gene therapies for various disorders. The major issues with current methods are toxicity to cells and a requirement for large amounts of reagents to achieve efficient transfection. The reverse transfection method developed in the Shea laboratory requires less nucleic acid and requires no additional reagents to attach them to the substrate. Increased transfection efficiency is achieved by seeding cells over the nucleic acid-bearing substrate, which raises the effective concentration of each molecule and facilitates its uptake by the cell. This approach also allows researchers to spatially control gene delivery by placing various nucleic acids in different portions of the solid substrate. This new gene delivery system could provide researchers and clinicians alike with a much-needed method for effective transfection, while decreasing toxicity and streamlining procedures.

TagsTHERAPEUTICS: gene therapy, research tool: reagent
Posted DateApr 21, 2015 12:16 PM


Tatiana Segura

Zain Bengali

Brian Anderson

Angela Pannier

Peter Chung

Jae-Hyng Jang

Lonnie D. Shea*


  • Gene therapy
  • Drug screening
  • Gene libraries for research use


  • Increases efficiency of transfection
  • Enables spatial control of gene delivery
  • Decreases toxicity to transfected cells
  • Expands the range of cells that can be transfected


IP Status

Issued US patent number 7,029,697

Marketing Contact

Michael Moore, PhD

Invention Manager

(p) 847.491.4645