Project TitleMethods for Yeast Cell-Free Protein Synthesis
Track Code2012-185, 2014-023, 2014-185
Short Description

Advanced method for cell-free protein production using a yeast platform

#researchtool #method #equipment #manufacture/processes #biomedical 


Northwestern University scientists have developed improved methods for cell-free protein synthesis (CFPS) for production of proteins and peptides in an industrial setting. Most CFPS platforms are generated from bacterial cell lysates, and this can cause expression problems for certain eukaryotic proteins. This invention, however, demonstrates that use of yeast cells (eukaryotes) can improve the yield and solubility of proteins that are produced by CFPS. The methods are scalable and inexpensive, unlike alternative eukaryotic cell based methods. Furthermore, these CFPS methods can be employed in both batch and semi-continuous reaction format, which makes it more versatile then current alternatives. It is expected that the yeast cell-free protein synthesis will enhance current efforts at large-scale production of therapeutic proteins and peptides as well as expand the range of functional genomic studies that can be performed. 

Tagsresearch tool: equipment, RESEARCH TOOL: method, MANUFACTURE/PROCESSES, biomedical
Posted DateMar 3, 2015 5:48 PM


Michael C. Jewett*

Charles Eric Hodgman

Rui Gan

Jennifer A. Schoborg

Mark J. Anderson

Benjamin James Des Soye

Seok Hoon Hung

Yong Chan Kwon

Rey William Martin

Jessica Carol Stark


  • Large scale production of proteins and peptides
  • Functional genomics studies
  • Improving other CFPS platforms


  • Scalable
  • Cost-effective
  • High yield of active protein (400-fold improvement over alternatives)
  • Simple 


IP Status

US and international patent applications have been filed. 

Contact Information

Gwendolyn Humphreys, PhD

Invention Associate

(p) 847.467.0308