Details

Project TitleReporter Mice for Tissue and Cell-Based Measurements of Circadian Rhythms
Track Code2008-105
Short Description

Animal model for PERIOD2 protein expression reporting

#researchtool #animalmodel

Abstract

Circadian rhythms are a popular area of research, and a number of proteins involved in the process have already been identified – including the PERIOD2 protein. Northwestern investigators have developed a mouse strain expressing a luciferase reporter gene fused to the C-terminus of the mouse Period 2 (Per2) gene to create a PER2::LUCIFERASE fusion protein. This fusion gene replaces the endogenous Per2 gene and enables scientists to follow the level of the PERIOD2 protein by performing luciferase assays. The protein can be followed in vivo in mice, from tissues taken from mice and explanted in culture, and from cells prepared from mouse tissues, such as mouse embryonic fibroblasts (MEFs) and skin fibroblasts. Circadian rhythms can subsequently be measured from mice, tissue explants, or cells using bioluminescent recordings or imaging. 

 
Tagsresearch tool: animal model
 
Posted DateFeb 27, 2013 1:53 PM

Inventor(s)

Seung-Hee Yoo

Joseph S. Takahashi*

Applications

• Research tool for the study of circadian rhythms

• System to study expression profile of PERIOD2 protein    

Advantages

• Allows for in vivo and ex vivo study

• Imaging or bioluminescent data can be collected from samples

Publications

IP Status

Contact Information

Becky Crump, PhD

Associate Director

(p) 847-491-3630

(e) beckyc@northwestern.edu