Details

Project TitleMultiple Myeloma Cell Lines with Either Glucocorticoid Sensitivity and Resistance
Track Code2008-100
Short Description

Tissue culture cell lines derived from the peripheral blood of a multiple myeloma patient.

#researchtool #cellline

Abstract

Northwestern researchers have created tissue culture cell lines derived from the peripheral blood of a multiple myeloma patient who had been treated with glucocorticoids and become resistant to that treatment.  The blood was separated on a Ficoll gradient to isolate the compartment containing the myeloma cells. These isolated cells were expanded in tissue culture and named MM.1 cells. The MM.1 parental cell line was further sub-cloned by limiting dilution to isolate cells that are sensitive to the killing effects of glucocorticoids. The resultant cells are named MM.1S.  The MM.1 parental cell line was also treated with glucocorticoids at increasing doses over time and the resultant cells were not killed by high doses of glucocorticoids. These cells are named MM.1R.  These cell lines have been widely used for the pre-clinical development of therapeutics designed to treat multiple myeloma. There are many other human myeloma cell lines in existence, but these cell lines are unique in that both glucocorticoid-sensitive and- resistant sub-clones were derived from the same patient. This is a very attractive model for the study of how glucocorticoid resistance develops in patients.

 
TagsRESEARCH TOOL: cell line
 
Posted DateJan 14, 2013 2:51 PM

Inventor(s)

Nancy Krett

Steven Rosen*

Applications

Model for development of MM resistance to glucocorticoids

Pre-clinical Development of Therapeutics for MM

Advantages

All cell lines are derived from same patient

Publications

Goldman-Leikin RE, Salwen HR, Herst CV, et al. (1980) Characterization of a novel myeloma cell line. Journal of Laboratory Clinical Investigation.  113:  335-45.

Moalli PA, Pillay S, Weiner D, Leikin R and Rosen ST (1992)  A mechanism of resistance to glucocorticoids in multiple myeloma: transient expression of a truncated glucocorticoid receptor mRNA.  Blood.  79:  213-22.

IP Status

Contact Information

Gwendolyn Humphreys, PhD

Invention Associate

(p) 847.467.0308

(e) gwendolyn.humphreys@northwestern.edu