Project TitleHigh-Throughput Imaging of Graphene Based Sheets by Fluorescence Quenching Microscopy
Track Code2009-099
Short Description

Method for visualing graphene without special substrates

#materials #graphene/graphite #instrumentation #microscopy


Graphene based sheets have stimulated great interest due to their superior mechanical, electrical and thermal properties. A general visualization method has been developed that allows quick observation of these single atomic layers which can greatly facilitate sample evaluation and manipulation, and provide immediate feedback to improve synthesis and processing strategies. Thus, graphene based sheets can be made highly visible under a fluorescence microscope by quenching the emission from a dye coating, which can be conveniently removed afterwards by rinsing without disrupting the sheets. Graphene, graphene oxide, or reduced graphene oxide sheets can be readily observed by eye with good contrast for layer counting. The fluorescence quenching mechanism eliminates the need for special substrates. Direct observation of suspended sheets in solution was also demonstrated. With photomasks, fluorescence quenching microscopy also allows in-situ photo lithography on individual graphene based sheets to be carried out on a common fluorescence microscope. The fluorescence quenching microscopy offers unprecedented imaging flexibility and should become a general tool for characterizing graphene based materials.

Tagsinstrumentation: microscopy, MATERIALS: graphene/graphite
Posted DateMar 19, 2011 3:15 PM


Laura Cote
Jiaxing Huang*
Franklin Kim
Jaemyung Kim


  • Microscopy imaging for characterization of graphene-based materials


  • High throughput, high contrast and high layer (thickness) resolution
  • Elimination of need for special substrates: works on plastic substrates
  • Capability of distinguishing graphene materials of different chemical properties such as for imaging-patterned graphene surfaces
  • Direct observation of graphene-based sheets in solution
  • Unprecedented imaging flexibility
  • Compatibility with common fluorescence microscopes for in situ photolithograph on individual sheets


IP Status

A patent application has been filed.

Contact Information

Sarah Kamper, PhD

Invention Associate

(p) 847.491.5095