Details

Project TitleRNA-Directed DNA Editing in Stem Cells
Track Code2013-081
Short Description

Simple, efficient and user-friendly approach for DNA editing in mammalian cells

#genomics #RNA #DNA #researchtool #DNA/RNAsynthesis

Abstract

Northwestern researchers have developed a method to accomplish genome editing in human pluripotent stem cells (hPSCs). This method uses ribonucleic acids (RNAs) as a guide for targeting genes of interest. Current methods are cumbersome and time-consuming. They require that a pair of proteins be designed, expressed and validated for each gene that is to be edited.Prof. Sontheimer and his colleagues have developed a simple and fast genome editing method that not only requires less reagents but also exhibits higher targeting efficiencies. This new technology offers a useful tool to discovery more stem cell-based therapies.

 
Tagsgenomics: DNA, GENOMICS: RNA, research tool: DNA/RNA synthesis
 
Posted DateAug 14, 2015 1:16 PM

Inventor(s)

Alfonso Mondragon
Eric Sontheimer*
Yan Zhang
Rakhi Rajan
Zhonggang Hou

James Thomson

Applications

• RNA-directed DNA cleavage and binding

• RNA-directed genome editing in live cells

Advantages

• Simple and time-efficient

• User-friendly

Publications

IP Status

US and international patent applications have been filed.

Marketing Contact

Michael Moore, PhD
Invention Manager  

(p) 847.491.4645

(e) michaelmoore@northwestern.edu